Target- Carbonic Anhydrase (Salmonella)

ORGANISM:Salmonella [enterica subsp. enterica serovar Enteritidis str. P125109]

GENE of INTEREST: Carbonic Anhydrase (PDB #3QY1)

BACKGROUND/ DISEASE

IMAGE:

NCBI GENE # or RefSeq#: 1251689 (undetermined essentiality)

EC# OF ENZYME: 4.2.1.1

CDS GENE SEQUENCE:
>gi|16763390:c202072-201410 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 chromosome, complete genome
ATGAAAGACATAGATACACTCATCAGCAACAATGCACTATGGTCAAAAATGCTGGTGGAAGAGGACCCCGGATTTTTTGAGAAACTGGCGCAAG
CGCAAAAACCGCGCTTTCTATGGATTGGATGTTCCGACAGCCGCGTTCCCGCAGAGCGTTTAACCGGGCTTGAACCGGGCGAATTGTTTGTTCA
CCGTAATGTGGCTAACCTGGTTATTCACACCGATCTGAACTGTCTCTCCGTGGTTCAGTATGCGGTAGATGTTCTGGAAGTTGAGCATATTATCATT
TGCGGCCACTCCGGTTGCGGCGGTATCAAGGCTGCGGTAGAAAACCCTGAGCTGGGCTTGATTAATAACTGGCTGCTGCATATCCGCGACATCTG
GCTTAAACATAGCTCGCTGTTGGGAAAAATGCCCGAAGAGCAACGTCTGGACGCGCTCTATGAATTGAACGTCATGGAACAGGTCTATAACCTGG
GGCATTCCACCATTATGCAGTCAGCGTGGAAACGCGGTCAGAATGTGACCATTCACGGCTGGGCGTACAGTATCAATGATGGCCTGCTGCGCGAT
CTTGACGTCACCGCCACCAACAGAGAAACGCTGGAGAACGGCTATCATAAGGGTATTTCCGCCCTAAGTCTGAAATACATTCCCCACCAATAA


OPTIMIZED GENE SEQUENCE USED IN LAB:

>filtered DNA sequence consisting of 663 bases.

ATGAAAGACATCGACACCCTGATCTCTAACAACGCGCTCTGGTCTAAGATGCTGGTTGAA

GAAGACCCGGGCTTCTTCGAGAAACTGGCGCAGGCGCAGAAACCGCGTTTCCTGTGGATC

GGTTGCTCTGACTCTCGTGTTCCGGCGGAACGTCTGACTGGTCTGGAACCGGGTGAACTG

TTCGTTCACCGTAATGTTGCGAACCTGGTTATCCACACCGACCTGAACTGCCTGTCTGTT

GTTCAGTACGCAGTTGATGTTCTGGAAGTTGAACACATCATCATCTGCGGTCACTCTGGT

TGCGGTGGTATCAAAGCGGCGGTTGAAAACCCGGAACTGGGCCTGATTAACAACTGGCTG

CTGCACATCCGTGACATCTGGCTGAAACACTCTTCTCTGCTGGGTAAAATGCCGGAAGAA

CAGCGTCTGGACGCGCTGTACGAGCTGAACGTAATGGAGCAGGTTTACAACCTGGGCCAC

TCTACCATCATGCAGTCTGCGTGGAAACGTGGTCAGAACGTTACCATCCACGGTTGGGCG

TACTCTATCAACGACGGTCTGCTGCGTGACCTGGACGTTACTGCGACCAATCGTGAAACC

CTGGAAAACGGTTACCACAAAGGTATCTCTGCGCTGTCTCTGAAATACATCCCGCACCAG

TAA


AMINO ACID SEQUENCE:
>gi|16763561|ref|NP_459176.1| carbonic anhydrase [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]
MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSDSRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHS
GCGGIKAAVENPELGLINNWLLHIRDIWLKHSSLLGKMPEEQRLDALYELNVMEQVYNLGHSTIMQSAWKRGQNVTIHGWAYSINDGLLRDLDVTA
TNRETLENGYHKGISALSLKYIPHQ

PDB: http://www.pdb.org/pdb/explore/remediatedSequence.do?structureId=3QY1